gfp rab7 t22n (Addgene inc)
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Addgene inc
gfp rab7 t22n
Gfp Rab7 T22n, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rab7+t22n/EGFP-Rab7A+T22N+(Plasmid+%2328048)/pmc12583604-31-0-3
Average 92 stars, based on 17 article reviews
Gfp Rab7 T22n, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rab7+t22n/EGFP-Rab7A+T22N+(Plasmid+%2328048)/pmc12583604-31-0-3
Average 92 stars, based on 17 article reviews
gfp rab7 t22n - by Bioz Stars,
2026-09
92/100 stars
Images
1) Product Images from "BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM"
Article Title: BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM
Journal: The EMBO Journal
doi: 10.1038/s44318-025-00543-9
Figure Legend Snippet: ( A ) Fluorescence images of RPE-1 cells expressing exogenous BLTP3A-mRFP (shown in inverted grays) and (not shown) GFP-tagged wild-type Rab7 (left) or dominant negative (DN) Rab7 (T22N) (right). Scale bar, 5 μm. ( B ) CLEM of a BLTP3A-mRFP positive cluster in an RPE-1 cell expressing GFP-tagged dominant negative Rab7. Left: fluorescence image of BLTP3A-mRFP (magenta). Scale bar, 1 μm. Right: EM micrograph of the field shown at left revealing that the BLTP3A-mRFP fluorescence reflects clusters of small vesicles. Scale bar, 500 nm. ( C ) BLTP3 chimeras design. Left: Surface representation of the predicted RBG core of BLTP3A. Red and blue indicate positive and negative charges, respectively, and gray indicates hydrophobic surfaces. Right: Surface representation (top) and ribbon representation (bottom) of the “untwisted” protein showing individual RBG motifs. Bottom: Cartoon of chimeras consisting of BLTP3A (dark orange) and BLTP3B (light orange) RBG motifs. ( D ) High-magnification live fluorescence images of RPE-1 cells expressing the indicated BLTP3-mRFP constructs (magenta) and LAMP1-GFP (green). Individual channels are shown as inverted grays. Scale bar, 1 μm. ( E ) Ribbon representation of the AlphaFold prediction of a.a. 1–336 of BLTP3A. Blue indicates loops connecting adjoining RBG motifs, and gray indicates the first beta-strand of the third RBG motif. ( F ) Fluorescence images (inverted grays) of RPE-1 cells expressing BLTP3A-1-336-mRFP and either (not shown) GFP-Rab7 (left), or GFP-Rab7 T22N (right). Scale bar, 5 μm. A zoom of an area of the cell at left (dotted square) expressing BLTP3A-1-336-mRFP (magenta) is also shown, along with the Rab7 fluorescence (green), demonstrating the localization of BLTP3A-1-336-mRFP around the entire profile of lysosomes. Individual channels are shown as inverted grays. Scale bar, 2 μm. ( G ) Cartoon depicting the proposed association of BLTP3A vesicle clusters with the surface of lysosomes and the dependence of this association on Rab7.
Techniques Used: Fluorescence, Expressing, Dominant Negative Mutation, Construct
Related Articles
other:Article Title: BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM Article Snippet: Article Title: Endosomal trafficking participates in lipid droplet catabolism to maintain lipid homeostasis Article Snippet: Plasmid Preparation:Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons. Article Snippet: .. The following plasmids were used: mito-DsRed2 (a gift from T. Schwarz, Harvard Medical School), EGFP–FMRP (gift from G. Bassell, Emory University), GFP-POLG2 (gift from W. Copeland, NIH), Halo–FMRP (subcloned from EGFP–FMRP into pFN21A-HaloTag-CMV vector from Promega), COX8A–BFP (Goldsmith et al.49), LAMP1–Halo (Gallagher and Holzbaur50), GFP–MFF (Addgene, 49153), GFP-RAB7 (Addgene, 12605), Article Title: FMRP regulates MFF translation to locally direct mitochondrial fission in neurons Article Snippet: .. The following plasmids were used: mito-DsRed2 (a gift from T. Schwarz, Harvard Medical School), EGFP–FMRP (gift from G. Bassell, Emory University), GFP-POLG2 (gift from W. Copeland, NIH), Halo–FMRP (subcloned from EGFP–FMRP into pFN21A-HaloTag-CMV vector from Promega), COX8A–BFP (Goldsmith et al. ), LAMP1–Halo (Gallagher and Holzbaur ), GFP–MFF (Addgene, 49153), GFP-RAB7 (Addgene, 12605), Article Title: Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux Article Snippet: C18orf8 and CCZ1 532 cDNAs were purchased from Origene, followed by Gateway cloning into the pHAGE-C-533 terminal-FLAG-HA vector (C18orf8), pHAGE-N-terminal-FLAG-HA or pHAGE-GFP vector 534 (CCZ1). .. GFP-RAB7 WT , GFP-RAB7 Q67L , and Dominant Negative Mutation:Article Title: Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux Article Snippet: C18orf8 and CCZ1 cDNAs were purchased from Origene and subjected to Gateway cloning into the pHAGE-C-terminal-FLAG-HA vector (C18orf8), pHAGE-N-terminal-FLAG-HA, or pHAGE-GFP vector (CCZ1). .. GFP-RAB7 WT , GFP-RAB7 Q67L , and Article Title: Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux Article Snippet: C18orf8 and CCZ1 532 cDNAs were purchased from Origene, followed by Gateway cloning into the pHAGE-C-533 terminal-FLAG-HA vector (C18orf8), pHAGE-N-terminal-FLAG-HA or pHAGE-GFP vector 534 (CCZ1). .. GFP-RAB7 WT , GFP-RAB7 Q67L , and |